Home > Sandwich ELISA Kits > Human Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) ELISA Kit

Product Name:

Human Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) ELISA Kit

Cat.#:

JL19743

Regulatory Status:

RUO

Alternative:

CCL3-L1; LD78; SCYA3L; SCYA3L1; MIP1AP; MIP1-Alpha-P; Small Inducible Cytokine A3-Like 1; Macrophage Inflammatory Protein 1-Alpha-P; Tonsillar Lymphocyte LD78 Beta

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

78.12-5000pg/mL

Sensitivity:

27.65pg/mL

Specificity:

This kit recognizes Human CCL3L1 in samples. No significant cross-reactivity or interference between Human CCL3L1 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| CCL3L1

Chemokine (C-C motif) ligand 3-like 1, also known as CCL3L1, is a protein which is encoded by the CCL3L1 gene.This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. Specifically, chemokines attract lymphocytes to sites of infection or damage. This protein binds to several chemokine receptors including chemokine binding protein 2 (CCBP2 or D6) and chemokine (C-C motif) receptor 5 (CCR5).

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (pg/mL)	5000	2500	1250	625	312.5	156.25	78.12	0
OD	2.45	1.46	0.89	0.57	0.39	0.28	0.23	0.09
Corrected OD	2.36	1.37	0.8	0.48	0.3	0.19	0.14	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human CCL3L1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	94-108	103

| Linearity

Samples were spiked with high concentrations of Human CCL3L1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	89-96	90-110
1 : 2	Average (%)	91	94	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	98	108